Download e-book for iPad: Biochemistry of Smooth Muscle Contraction by Michael Barany

By Michael Barany

This necessary source presents a scientific account of the biochemistry of gentle muscle contraction. As a finished consultant to this quickly growing to be quarter of analysis, it covers the constitution and attribute homes of contractile and regulatory proteins, with distinctive emphasis on their envisioned functionality within the dwell muscle. additionally integrated during this publication are intermediate filament proteins, and desmin and vimentin, whose functionality in gentle muscle is unknown; and a number of other enzymes concerned about the phosphorylation-dephosphorylation of contractile and different proteins

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A I - - - S G - L L . . . . . - - - - - - o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o A o o o o o o o o o o o o o o o o FIGURE 7 Diagrammatic representation of the smooth muscle HC to show the location of the 7-amino-acid insert. The location of the 7-amino-acid insert in chicken gizzard and rabbit and rat visceral HCs is the same as for the 10- or 16-amino-acid inserts found in chicken and Xenopus nonmuscle HC-B, respectively. The amino acids surrounding the inserts are conserved in both smooth muscle and nonmuscle HCs.

1993a). Instead, all three segments of the myosin head are intertwined in space and the two proteolytically sensitive sites are located in separate surface loops. _ FIGURE 2 Separationof HC204and HC200by protein G-HPLC immunoaffinity chromatography. The three panels show Coomassie blue-stained SDS-5% polyacrylamidegels of bovine aorta myosin before (left panel) and following HPLC chromatography (right two panels). The antibodies used in this purification were raised to the amino acid sequence DADFNGTKSSE,which is at the carboxyl terminal of the HC204.

S _ _ _ Chicken NMHC-B - - - R - - H N - P P E S P K P V K H ....... Xenopus NMHC-B . . . H T - P ~ . . A I - - - S G - L L . . . . . - - - - - - o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o A o o o o o o o o o o o o o o o o FIGURE 7 Diagrammatic representation of the smooth muscle HC to show the location of the 7-amino-acid insert. The location of the 7-amino-acid insert in chicken gizzard and rabbit and rat visceral HCs is the same as for the 10- or 16-amino-acid inserts found in chicken and Xenopus nonmuscle HC-B, respectively.

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