Download e-book for kindle: Cellular and Molecular Effects of Mineral and Synthetic by Andrew Churg (auth.), John M. G. Davis, Marie-Claude Jaurand

By Andrew Churg (auth.), John M. G. Davis, Marie-Claude Jaurand (eds.)

Presented listed here are fresh info at the mechanisms of motion of alternative dusts and fibres of business curiosity. Emphasis is put on using mobilephone and organ tradition and lavage mobilephone populations received from guy and laboratory animals to clarify mobile and molecular occasions taking place after their interplay with fibrous and non-fibrous particulates together with steel compounds. In 4 sections, the amount offers examine findings within the following components: - mobile and Metabolic adjustments brought on by Mineral Dusts - Molecular adjustments and DNA adjustments Produced through Mineral Dusts - In Vivo Dust-Related Pathological techniques. Correlations among in Vitro and in Vivo facts - Physico-Chemical homes of Minerals in terms of Their organic Effects.

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Biochem. Biophys. 278: 60-64. Lund, LG, and Aust, AE. (1991) Mobilization of iron from crocidolite asbestos by certain chelators results in enhanced crocidolite-dependent oxygen consumption. Arch. Biochem. Biophys. 287: 91-96. Maltzman, W, and Czyzyk, L. (1984) UV irradiation stimulates levels of p53 cellular tumor antigen in nontransformed mouse cells. Mol. Cell BioI. 4: 1689-1694. Maples, KR, and Johnson, NF. (1992) Fiber-induced hydroxyl radical formation: Correlation with mesothelioma induction in rats and humans.

Science 247:294-301, 1990. Rahman Q and Casciano DA. Involvement of superoxide radical in the toxicity of mineral fibers. In: In Vitro Effects of Mineral Dusts. Springer-Verlag, Berlin, Germany, pp483-488, 1985. 21 Vallyathan V, Mega JF, Shi X and Dalal NS. Enhanced generation of free radicals from phagocytes induced by mineral dusts. Am J Respir Cell Mol Bioi 6:404-413, 1992. Vallyathan V, Schwegler D, Reasor M, Stettler L, Clere J and Green FHY. Comparative in vitro cytotoxicity and relative pathogenicity of mineral dusts.

0 x 105 cells/mL by dilution with RPM I 1640 media. p53 Expression: Lung epithelial cells which were originally derived from normal F344 rats as described by Li et al. (1983) were cultured for 48 hours so that the LEC were 70-80% confluent prior to treatment. Cells were cultured in 225 cm 2 tissue culture flasks in RPMI 1640 medium containing 10% newborn calf serum. The culture flasks were divided into three groups. 1 pg/cm 2 ) for 4 hours after which the cells were washed in PBS and harvested by trypsinization, pelleted by centrifugation, and resuspended in PBS.

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