Download e-book for iPad: Cellular Pathology Technique by C. F. A. Culling

By C. F. A. Culling

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Micr. , 97, 621-623 BOSMAN, F. , KUiPER,c, VAN DER WAAL, A. and KREUNIG, J. (1977). The influence of fixation on immunoperoxidase staining of plasma cells in paraffin sections of intestinal biopsy specimens. Histochemistry, 53, 57-62 BOWES, J. H. (1963). A fundamental study of the mechanism of deterioration of leather fibres. Br. Leather Manuf. Res. Assoc. Report. BOWES, J. A. and CATOR, C. W. (1967). The reaction of glutaraldehyde with proteins and other biological materials. J. R. Micr. Soc, 85, 193-200 CHAMBERS, R.

Chromic acid hydrolyses DNA with the conversion of its pentose sugar to an aldehyde; it also converts carbohydrates to aldehydes. DNA and carbohydrates will 34 Fixation therefore give positive staining with Schiff reagent without the normal pretreatment by periodate or HC1 associated with the PAS or Feulgen reactions respectively. Tissues fixed in chromic-acid-containing fixatives will require thorough washing for the same reason as those fixed in potassium dichromate. Picric acid (C 6 H 2 (N0 2 ) 3 OH) Picric acid is supplied in water and should be kept under water.

Because of this, and the great shrinkage caused, mercuric chloride is seldom used alone; it is a constituent of many good routine fixatives when combined with reagents which combat these defects, such as acetic acid, formalin, potassium dichromate, and so on. Fixatives containing mercuric chloride should be listed among the intolerant fixatives, in that exposure of tissues to their action in excess of recommended times, will produce excessive hardness and make the cutting of thin sections difficult.

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