Download e-book for kindle: Dictionary of DNA and Genome Technology by Paul Singleton

By Paul Singleton

DNA know-how has a necessary function in varied fields similar to legal research and gene remedy – dynamic parts regarding many specialized phrases and strategies. This distinctive dictionary deals present, exact, available info to teachers, researchers, scholars and technicians through the biomedical and similar sciences.

  • The new version represents an incredible replace with 50% extra entries at the very newest advancements.
  • Clear reasons of phrases, concepts, and exams, together with advertisement structures, with targeted assurance of many vital systems and techniques.
  • Includes well-established method plus new know-how and knowledge from the newest study journals, going well past the remit of so much technological know-how dictionaries.
  • Essay-style entries on many significant subject matters to aid beginners to the sphere.
  • Covers themes proper to drugs (diagnosis and gene therapy); veterinary technological know-how; biotechnology; biochemistry; pharmaceutical science/drug improvement; molecular biology; microbiology; epidemiology; genomics; environmental technological know-how; plant science/agriculture; taxonomy; and forensic technological know-how.

 

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Thus, splicing of pre-mRNA of gene bcl-x normally produces a long anti-apoptotic mRNA as well as a short pro-apoptotic mRNA, and the ratio of these two mRNAs in the cell is a key determinant of cancer progression. By using a short antisense PNA conjugated to an oligopeptide containing eight serine– arginine repeats (cf. SR PROTEINS), it was possible to modify the splicing pattern so that the short (pro-apoptotic) mRNA became the dominant influence; this procedure was able to produce APOPTOSIS in HeLa (cancer) cells [Nucleic Acids Res (2005) 33(20):6547–6554].

G. caspase-mediated activation of the nuclease caspase-activated DNase (CAD) results in DNA fragmentation. ) Caspase activation apparently occurs primarily by cleavage of the zymogen. In at least some cases, one caspase may be activated by another, ‘upstream’ caspase in a caspase cascade. In macrophages that have taken up the bacterium Shigella flexneri (by phagocytosis), apoptosis in the macrophage can be promoted by a plasmid-encoded protein, IpaB, secreted by S. flexneri; IpaB activates the host’s IL-1b-converting enzyme (ICE, a cysteine protease) which then initiates apoptosis.

In some cases two primers bind with relative efficiency, on opposite strands, at locations separated by a few hundred bases. If synthesis can occur normally from these two primers, a further round of cycling under low-stringency conditions, followed by many cycles under high-stringency conditions, may produce copies of an amplicon delimited by the two best-fit sequences. In the phase of high-stringency cycling not all the primers will bind to their best-fit sequences – so that only a proportion of the amplicons produced under low-stringency conditions will be amplified in the high-stringency phase.

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